# Illumina Sequencing From Deep Sea Sediments # PI: Peter Girguis (Harvard) # Contributor: Melissa Adams # Version date: 12 October 2018 ========================= sequence_accession_number link Species_Names description_of_the_types_of_sequences locations_where_species_were_collected latitude_dms longitude_dms latitude longitude Vessel Dive_number sequencing_and_analysis_methods instrument_and_model Analysis_methods ------------------------- SRX4556790 https://www.ncbi.nlm.nih.gov/sra/SRX4556790[accn] cold seep metagenome metagenomic sequencing of pushcore samples from hydrothermal vents Hydrate Ridge 44 34 10.20 N 125 8 48.48 W 44.5694 -125.1467 ROV Ropos 1458 PCR amplicon (from Archaea and Bacteria 16S universal primers 515F/806R) Illumina MiSeq with Reagent Kit v3 Raw sequences generated using manufacturer's software and then quality filtered using QIIME V. 1.8.0 SRX4556789 https://www.ncbi.nlm.nih.gov/sra/SRX4556789[accn] cold seep metagenome metagenomic sequencing of pushcore samples from hydrothermal vents Middle Valley 48 27 26.40 N 128 42 30.60 W 48.45722 -128.70861 DSV Alvin 4625 PCR amplicon (from Archaea and Bacteria 16S universal primers 515F/806R) Illumina MiSeq with Reagent Kit v3 Raw sequences generated using manufacturer's software and then quality filtered using QIIME V. 1.8.0 SRX4556788 https://www.ncbi.nlm.nih.gov/sra/SRX4556788[accn] cold seep metagenome metagenomic sequencing of pushcore samples from hydrothermal vents Guaymas Basin 27 0 27.84 N 111 24 27.84 W 27.0078 -111.4078 DSV Alvin 4486 PCR amplicon (from Archaea and Bacteria 16S universal primers 515F/806R) Illumina MiSeq with Reagent Kit v3 Raw sequences generated using manufacturer's software and then quality filtered using QIIME V. 1.8.0